ABSTRACT
ObjectiveTo observe the effect of Qinggan Jianpi Huoxue prescription（QGJPHXP） on the polarization of M1/M2 macrophages in rats with hepatic fibrosis induced by carbon tetrachloride（CCl4）. MethodA rat hepatic fibrosis model was established by intraperitoneal injection of 40% CCl4-olive oil suspension twice a week at the dosage of 2.0 mL·kg-1 for 8 weeks. After the model was successfully established， these rats were randomly divided into the model group， QGJPHXP group（32.084 g·kg-1） and Biejiajian pills（BJJP） group（0.925 5 g·kg-1）， with 12 rats in each group. The blank group was injected intraperitoneally with the same amount of olive oil. The rats in the administration groups were given the corresponding solution according to the dose， and the blank and model groups were given the same dose of purified water， once a day. After 4 weeks of continuous administration， the liver tissues of rats were taken and stained with hematoxylin-eosin（HE） and Masson to observe the pathological changes. The serums were collected to detect the alanine aminotransferase（ALT） and aspartate aminotransferase（AST） levels. Interleukin（IL）-6， IL-12， IL-10， IL-1β， transforming growth factor-β1（TGF-β1） and tumor necrosis factor-α（TNF-α） levels in liver tissues were measured by enzyme-linked immunosorbent assay（ELISA）. The expression levels of CD86 and CD206 were detected by immunohistochemistry（IHC）. Western blot and real-time fluorescence quantitative polymerase chain reaction（Real-time PCR） were used to detect the protein and mRNA expression levels of inducible nitric oxide synthase（iNOS）， arginase-1（Arg-1）， phosphorylated p38 mitogen-activated protein kinase（p-p38 MAPK）， nuclear transcription factor-κB p65（NF-κB p65） in liver tissues of rats. ResultCompared with the blank group， the hepatic cell plate was irregularly arranged, and local inflammatory cell infiltration and fibrous hyperplasia were observed， while the serum levels of ALT and AST were significantly increased in the model group（P<0.01）， and IL-1β， IL-6， IL-12， TGF-β1， TNF-α， CD86， CD206， iNOS， p-p38 MAPK，p38 MAPK and NF-κB p65 levels in liver tissues were obviously increased（P<0.05， P<0.01）， while the levels of IL-10 and Arg-1 were obviously decreased（P<0.05， P<0.01）. Compared with the model group， QGJPHXP group reduced the degree of liver cell fibrosis，and serum levels of ALT and AST（P<0.01）， and IL-1β， IL-6， IL-12， TGF-β1， TNF-α， CD86， iNOS， p-p38 MAPK， p38 MAPK， and NF-κB p65 levels in liver tissues were obviously decreased（P<0.05， P<0.01）， the levels of IL-10， CD206 and Arg-1 were obviously increased in the QGJPHXP group（P<0.05， P<0.01）. ConclusionQGJPHXP has ability to inhibit the activation of pro-inflammatory M1 macrophages， induce the secretion of anti-inflammatory cytokines by M2 macrophages， reduce the release of pro-fibrogenic cytokines， and promote the macrophage polarization of M1 to M2 in liver for tissue repair， thereby serving as an anti-inflammatory and anti-hepatic fibrosis drug.
ABSTRACT
To systematically evaluate the effect of traditional Chinese medicine（TCM） on the expression of inflammatory factors in peripheral blood of patients with coronary heart disease complicated with anxiety and depression，and explore its efficacy and safety in treatment of anxiety and depression. In this study，CNKI，VIP database，WanFang database，PubMed and Cochrane Library were searched to collect randomized controlled trials（RCTs） of TCM in the treatment of coronary heart disease complicated with anxiety and depression，and 2 researchers independently screened the literatures and extracted the data. The quality of the included literatures was evaluated with Cochrance bias risk evaluation tool and Meta analysis was conducted by Cochrane Revman 5.3 software. A total of 21 research articles were included，with a total sample size of 2 342 cases，1 175 cases in the treatment group and 1 167 cases in the control group. Meta analysis results showed that the treatment group reduced the hypersensitive C-reactive protein（hs-CRP）［standard mean difference（SMD）=-1.61，95% confidence interval（CI）（-2.14，-1.09），P<0.01］，interleukin（IL）-8［mean difference（MD）=-5.03，95% CI（-8.37，-1.70），P=0.003］，IL-17［MD=-33.27，95% CI（-40.15，-26.39），P<0.01］，tumor necrosis factor（TNF）-α［SMD=-1.18，95% CI（-1.98，-0.38），P<0.01］，and homocysteine（Hcy）［MD=-3.45，95% CI（-4.85，-2.04），P<0.01］. The treatment group was better than the control group in terms of relieving anxiety and depression，i.e. scores of Hamilton anxiety scale（HAMA） ［SMD=-1.97，95% CI（-2.48，-1.46），P<0.01］，Hamilton depression scale（HAMD） ［SMD=-1.94，95% CI（-2.50，-1.38），P<0.01］，and self-rating depression scale（SDS）［SMD=-0.72，95% CI（-0.90，-0.54），P<0.01］，so in terms of ，with statistically significant difference. 4 articles mentioned that no obvious adverse reactions occurred，4 articles mentioned that the treatment group had drowsiness，dry mouth and bitter mouth，gastrointestinal reactions，but the incidence rates were significantly lower than those of the control group. The other 13 articles did not mention the occurrence of adverse reactions.
ABSTRACT
Objective To investigate the effect of the laparotomy and laparoscopic surgery on the stress parameters and complication of patients with gastric cancer. Methods A total of 96 patients diagnosed as gastric cancer and treated by surgery in our hospital from January 2015 to January 2017 were divided into open operation group and laparoscopy group according to the operation method,48 cases in each group. Compared the operation time,bleeding volume,dissected lymph node number,postoperative hospitalization duration and anus exhausting time and complications in 6 months after surgery. The levels of WBC,CRP,TNF-α, IL-6 in serum before and after operation were detected by enzyme - linked immuno sorbent assay and compared. Results Compared with the open operation group, the bleeding volume,postoperative hospitalization duration and anus exhausting time of laparoscopy group were better with less dissected lymph node number and longer operation time, the differences were extremely significant(P ＜ 0. 01); the WBC,CRP,TNF-α, IL-6 levels of laparoscopy group at 1 day after the operation were lower than those of open operation group(P ＜ 0. 05). The incidence of complication of laparoscopy group was 22. 8％, which was less than 54. 7％ of control group, the difference was significant(P ＜ 0. 05). Conclusion Compared with the traditional open operation, laparoscopic radical gastrectomy can shorten the hospital stays and reduce the intraoperative blood loss, the stress response and complication rate after operation.
ABSTRACT
Objective To investigate whether (-)-epicatechin plays a role in neurological repair on traumatic brain injury in mice.Methods The mice model of traumatic brain injury was established by modified weight drop method.Experimental mice were randomly (random number) divided into the injury+ (Veh) group and the injury + (-)-epicatechin (EC) group.At 3 days after operation,the expression of IL-1β and TNF-α were detected.The number of necrotic cells of the lesion area was detected by PI staining.At 28 days after SCI,Morris Water Maze test was performed to observe the ability of spatial learning and memory in mice.The expression of neurotrophic factors BDNF and NGF were examined by qRT-PCR.The expression of NeuN was detected by immunofluorescence staining.EdU staining was used to observe the neurogenesis in the SGZ region.Results Compared with the Veh group,EC treated group showed better spatial learning and memory ability in time spent in correct quadrant at day 27 and 28 [24 d:(26.333±5.037)％ vs (26.583±5.802)％,P=0.938;25 d (33.300±4.724)％ vs (29.767±3.347)％.P=0.166;26 d:(41.017±7.246)％ vs (32.800±8.145)％,P=0.095;27 d:(48.017±7.424)％ vs (35.267±6.748)％,P=0.011;28 d:(51.617±9.017)％ vs (41.116±6.467)％,P=0.043] and in latency to platform at day 27 and 28 [24 d:(62.967±5.494) s vs (63.917±7.027) s,P=0.800;25 d:(50.533±10.305) s vs (57.217±13.085) s,P=0.349;26 d:(40.333± 10.526) s vs (50.133±11.039) s,P=0.147;27 d:(28.717±4.137) s vs (44.533±7.181) s,P=0.001;28 d:(21.950±6.889) s vs (37.567±5.974) s,P=0.002].There was a decreased expression of IL-lβ and TNF-α and increased level of neurotrophic factor BDNF and NGF after EC treatment in EC treatment group,compared to the veh treatment group [IL-1β and TNF-α:(42.690±3.057) ng/mL and (750.167±51.941) ng/mL vs (71.670±4.996) ng/mL and (1 085.167±68.535) ng/mL,P=0.000 6 and 0.003;BDNF and NGF:0.543±0.033 and 0.334±0.041 vs 0.756±0.088 and 0.514±0.047,P=0.048 and 0.017)].EC decreased the cell death near injury area (54.833±5.486 vs 74.000±5.323,P=0.031),increased NeuN positive cells (76.667±6.386 vs 42.167±5.237,P=0.002),and increased neurogenesis in SGZ area (12.667±0.760 vs 7.500±1.258,P=0.031).Conclusions (-)-Epicatechin plays an important role in functional recovery after traumatic brain injury in mice.The underlying mechanisms are closely related to inhibited inflammation,enhanced neurotrophic factors and improved neurogenesis.
ABSTRACT
Objective To investigate the effect of enteral nutrition and probiotics on inflammatory factors and immune function in aplastic anemia (AA) rats.Methods AA rats model were established by intraperitoneal injection of fluorouracil and lavage Maryland,and the successful AA rats model were randomly divided into model group,enteral nutrition group,probiotics group and combination group,10 rats in each group.Another 10 Wistar rats were selected as blank group.Rats in the blank group and model group were given saline lavage (same volume 0.9％ NaC1),rats in the enteral nutrition group were given Peptisorb nutrient solution [10 g/(kg· d)] by lavage,rats in the probiotics group were given live Clostridium butyricum [2 g/(kg · d)] by lavage,and rats in the combination group were given Peptisorb nutrient solution [10 g/(kg · d)] and live Clostridium butyricum [2 g/(kg · d)] by lavage for 4 weeks.The pathological structure of the bone marrow were observed by HE staining,the contents of white blood cells (WBC),red blood cell (RBC),platelet (PLT) and hemoglobin (Hb) in blood cell were detected by analyzer,the levels of serum tumor necrosis factor alpha (TNF-α),interleukin-2 (IL-2) and interleukin-11 (IL-11) were detected by ELISA method,and the contents of CD4+,CD8+ and NKT cells were detected by flow cytometry instrument.Results The contents of WBC,RBC,PLT and Hb in the model group were significantly lower than that in the blank group,the contents of WBC,RBC,PLT and Hb in enteral nutrition group,probiotics group and combination group were significantly higher than that in the model group,and the combination group was better than the enteral nutrition group and probiotics group (P＜0.05),but the enteral nutrition group and probiotics group had no statistically significant difference (P＞0.05).The level of TNF-o and IL-2 were significantly higher and the IL-11 level was significantly lower in the model group than in the blank group,the level of TNF-α and IL-2 were significantly lower and IL-11 level were significantly higher in the enteral nutrition group,probiotics group and combination group than in the model group,and the combination group was better than the enteral nutrition group and probiotics group (P＜0.05),but the enteral nutrition group and probiotics group had no statistically significant difference (P＞0.05).The contents of CD4+ and NKT cells were significantly lower and the CD8+ cells was significantly higher in the model group than in the blank group,the level of CD4+ and NKT cells were significantly higher and CD8+ cells were significantly lower in enteral nutrition group,probiotics group and combination group than in the model group,and the combination group was better than the enteral nutrition group and probiotics group (P＜0.05),but the enteral nutrition group and probiotics group had no statistically significant difference (P＞ 0.05).Conclusion Compared with normal consumption or oral glucose,the enteral nutrition combined with probiotics can reduce inflammatory response and improve bone marrow hematopoietic function and immune function in AA rats.
ABSTRACT
Objective@#To explore the effect of neurokinin-1 receptor small interfering RNA (NK-1R-siRNA) on the expression of inflammation factors in allergic rhinitis (AR).@*Methods@#Twenty-four male SD rats were divided into three groups randomly (by random number table methord): NK-1R-siRNA group, negative control siRNA (NC-siRNA) group and saline group, with 8 rats in each group. SD rats were sensitized and challenged with ovalbumin (OVA) to induce AR. The rats were treated intranasally with NK-1R-siRNA, NC-siRNA or saline before and during the challenge period. The AR symptoms were observed. The levels of OVA-specific IgE were measured by enzyme-linked immunosorbent assay (ELISA). The levels of NK-1R expression in the nasal mucosal tissues were determined by real time PCR (RT-PCR) and immunohistochemistry. Antibody array was used in studying the expression of inflammation cell factors in nasal mucosa. SPSS 11.0 software was used for one-factor analysis of variance.@*Results@#Compared with saline group, AR symptoms relived significantly in NK-1R-siRNA group (nose rubbing (31.4±8.9)/15 min vs (69.5±17.9)/15 min, sneezing (7.2±1.9)/15 min vs (23.7±9.2)/15 min, nasal secretions (7.1±2.3) mg vs (24.1±4.4) mg, t value was 38.100, 17.125, 16.837, respectively, all P<0.01), and the level of serum OVA-specific IgE was also reduced ((8.56±0.73) ng/ml vs (18.05±1.22) ng/ml, t=9.787, P<0.01). The RT-PCR and immunohistochemistry results showed that the expression of NK-1R in nasal mucosa of NK-1R-siRNA group was remarkably reduced than that of the NC-siRNA group and saline group. After the treatment of NK-1R-siRNA, the expression of interleukin (IL) 1α, IL-1β, IL-4, IL-6 and IL-13 decreased, while the interferon-γ (IFN-γ) and IL-10 increased.@*Conclusion@#NK-1R-siRNA could regulate the release of inflammation factors in AR nasal mucosa, thus relive the allergic inflammation.
ABSTRACT
Objective To research the clinical efficacy of Dialog vario inlay teeth adjacent surface tooth repair and the influence on inflammatory factor and oxidative stress molecules in gingival sulcus fluid.Methods From September 2012 to September 2015,104 patients with ortho dental defects treated in the hospital were divided into study group and control group,with 52 cases in each group,control group used e-Max inlay repair,the research group used Dialog vario inlay repair.Clinical curative effect,beta interleukins-1β,6,8 (IL-β,IL-6,IL-8),tumor necrosis factor-α (TNF-α),propylene glycol (MDA),superoxide disproportionation alcohol (SOD),glutathione peroxidase (GSH-Px),gum situation were compared between two groups.Results The replacement integrity,marginal consistency,color matching and secondary caries rate in the study group were superior to those of the control group (P＞ 0.05).IL-β,IL-6,IL-8,TNF-α,MDA of study group were lower than the control group (P＜0.05).SOD and GSH-Px of study group were higher than those of the control group(P＜0.05).Periodontal status of research group was better than that of control group (P＜0.05).Conclusion The clinical efficacy of Dialog vario inlay in the repair of ortho dental defects may be related to the reduction of inflammatory reaction in gingival crevicular fluid and oxidative stress.
ABSTRACT
Objective To observe the clinical efficacy of Jiangzhi Xiaoban Tables facilitating routine Western medicine therapy on coronary plaque and inflammatory factors in patients with coronary heart disease angina (CHDA) of blood stasis obstruction syndrome. Methods Totally 156 patients were divided into TCM group and control group with randomized parallel controlled method, with 78 cases in each group. The control group orally took aspirin enteric-coated tablets and trimetazidine hydrochloride tablets and received symptomatic treatment. On the basis of control group,TCM group received Jiangzhi Xiaoban Tablets,4 tablets per time,three times per day,for 12 weeks. Angina pectoris, TCM curative effect, electrocardiogram, TCM symptom scores, TPS, SSS, CADS and inflammatory factors before and after treatment in both groups were evaluated. Results 12 and 10 cases in TCM group and control group were lost, respectively. The total effective rate of angina pectoris was 89.39% (59/66) in the TCM group and 72.06% (49/68) in the control group. The total effective rate of TCM efficacy was 90.09% (60/66) in the TCM group and 67.65% (46/68) in the control group. TCM group better than the control group (P<0.05). Compared with before treatment, the numbers of ST segment down shift, T wave lowering and T wave inverting decreased in both groups (P<0.05). After treatment, the numbers of ST segment down shift, T wave lowering and T wave inverting in the TCM group were less than the control group (P<0.05). Compared with before treatment, SAQ scores in both groups increased, while TCM syndrome score decreased significantly. SAQ scores in the TCM group after treatment were higher than the control group, while TCM score were lower than the control group (P<0.05). Compared with before treatment, the TPS, SSS and CADS of the patients with blocking coronary lesions decreased in both groups (P<0.05). After treatment, the ratio of TPS, SSS and CADS of the patients with blocking coronary lesions in TCM group was lower than the control group (P<0.05). Compared with before treatment, the levels of hs-CRP, IL-6, TNF-α, TGF-β1 and MCP-1 decreased in both groups after treatment (P<0.05). The levels of hs-CRP, IL-6, TNF-α and TGF-β1 in the TCM group were better than the control group (P<0.05). Conclusion Jiangzhi Xiaoban Tables facilitating routine Western medicine therapy can stabilize coronary plaque of CHDA of blood stasis obstruction syndrome, which may realize cardiomyocyte protection by inhibiting inflammatory factors.
ABSTRACT
Objective: To explore influence of continuous thoracic paravertebral block(CTPVB)under ultrasound-guidance combined with general anesthesia on inflammatory reaction and homodynamic of patients who underwent thoracotomy.Methods: 138 patients who underwent thoracotomy were prospectively selected and were divided into observation group(69 cases)and control group(69 cases)as random number table.Patients of observation group received CTPVB under ultrasound-guidance combined with general anesthesia and that of control group received general anesthesia.The changes of IL-6,IL-10 and TNF-α postoperative 48h and postoperative VAS score between the two groups were compared.And the VAS scores postoperative 2h,12h,24h and 48h also were observed.Besides,the mean arterial pressure and heart rate between the two groups at various time point included tranquillization time(T0),the 15 min(T1)after CTPVB combined with general anesthesia,the time post induced intubation(T2),5 min post skin incision(T3)and the time pre extubation(T4)were compared and analyzed so as to research its influence on hemodynamic.Results: The IL-6 and TNF-α postoperative 48h of observation group were significantly lower than that of control group(t=15.95,t=46.99,P＜0.05).And postoperative IL-10 of observation group was significantly higher than that of control group(t=134.3,P＜0.05).And the VAS scores of observation group at postoperative 2h,12h,24h and 48h were significantly lower than that of control group(t=13.91,t=17.1,t=2.321,t=9.231,P＜0.05),respectively.Conclusion: CTPVB under ultrasound-guidance combined with general anesthesia can improve postoperatively analgesic effect for patients,and it don't affect patients' blood pressure and heart rate,and it can reduce inflammatory reaction of patients who underwent thoracotomy and promote rehabilitation of patients.
ABSTRACT
AIM:To investigate the influence of Jiedu hugan granule on the inflammatory factor and tight junction protein of small intestine in rats with immunological liver injury.METHODS:Fifty SD rats were randomly divided into control group,model group,low,middle and high experimental group of Jiedu hugan granule (2.7,5.4,and 10.8 g/kg).All the groups excepted the control group received intraperitoneal injections of 0.5 mL pig serum for each to establish immunological liver injury model.The experimental group was irrigated ltime/d,continuous dosing for 14 d.The liver tissue and small intestinal tissue pathological changes were observed by HE staining,the serum alanine aminotransferase (ALT),aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were detected,and the expression of liver tissue TNF-α and small intestine tissue occludin-5 were detected by immunohistochemical method.RESULTS:The model group liver tissue showed accumulation of fat cells,liver cells enlargement,disorganized liver and associated with inflammatory cells infiltration;the experimental group liver cell morphological ruled,hepatic cords in alignment and inflammatory cells infiltration significantly decrease.The model group small intestinal mucosa villi showed atrophy,fall off of epithelial cell,edema,large number of inflammatory cells infiltration;the experimental group showed closelyknitted small intestinal mucosa villi,relieve edema,and no obvious infiltration of inflammatory cells.The levels of ALT,AST,LDH and TNF-α in experimental group were statistically lower than the model group,and the levels were statistically reduce with the dose of jiedu hugan granule increased.The levels of occludin-5 in experimental group were statistically highter than those of the model group,and the levels of occludin-5 were statistically increase with the dose of Jiedu hugan granule increased,the differences were statistically significant (P ＜ 0.05).CONCLUSION:Jiedu hugan granule can improve the organizational structure of liver and small intestine,protect the damage of liver,reduce inflammation,and maintain small intestine mucosal barrier function.
ABSTRACT
Objective To explore the effect of Shenling Baizhu power on inflammatory factors and immune function for recurrent aphthous ulcer. Methods Ninety patients with recurrent aphthous ulcer in our hospital were divided into control group and study group according to random number table method, with 45 cases each group.The control group were given oral vitamin B2, levamisole and cetyl pyridinium chloride gargle solution therapy, and the study group were given Shenling Baizhu power on the basis of control group.After one week treatment, the clinical curative effect were observed, the serum inflammatory factors levels of tumor necrosis factor alpha ( TNF-α) , interleukin 2 ( IL-2 ) and interleukin 6 ( IL-6 ) were determined by Elisa method, the levels of peripheral blood T cell subsets CD3 +, CD4 +, CD8 +and natural killer cells( NKT) were determined by flow cytometry instrument, and the recurrence rate were observed after one year follow-up.Results After one week treatment, the total effective rate 91.30% in study group was significantly higher than the control group 69.57%, the difference was statistically significant(χ2 =5.874, P=0.015).The serum levels of TNF-α, IL-2 and IL-6 in study group were significantly lower than the control group, the difference was statistically significant(P<0.05).The levels of CD3 +, CD4 +, CD8 +and NKT in study group were significantly higher than the control group, the difference was statistically significant(P<0.05).Followed up for 1 year, the recurrence rate 17.78%(8/45) in study group was significantly lower than the control group 46.67%(21/45), the difference was statistically significant(χ2 =8.598, P =0.003).Conclusion The Shenling Baizhu power treatment of recurrent aphthous ulcer has better clinical curative effect, can reduce levels of inflammatory factor, improve immune function, and reduce the recurrence rate.
ABSTRACT
Aim To study the influence of rosuvastatin on toll-like receptor 4 ( TLR4 ) , and its downstream nu-clear transcription factor NF-kappa B p65, IκBα, in-flammation factors TNF alpha in rats with myocardial hypertrophy induced by pressure overload .Methods Myocardial hypertrophy was induced by abdominal aor-tic constriction ( AAC ) .Male Wistar rats were divided into 3 groups(n=10):① sham-operated rats(S);②AAC rats(M);③AAC+rosuvastatin(10 mg· kg -1· d-1 ) rats.From 1 week pre-opertion to 4 weeks post-operation, three groups were performed by gavage ad-ministration with equal volume of rosuvastatin and vehi-cle.The rats underwent cardiac color Doppler exami-nation.The level of ANP,TLR4, NF-κB p65,IκBα, TNF-αmRNA and protein expression in myocardium were detected by real-time PCR, Western blot, immu-nohistochemical and ELISA respectively .Results The sizes of heart and cardiomyocytes and the expression of ANP mRNA and TLR4 signaling molecules were signif-icantly increased in group M , which could be blocked by rosuvastatin .TLR4 protein was positively related to ANP, NF-κB p65 and TNF-αrespectively .Conclusion Rosuvastatin prevents cardiac hypertrophy induced by pressure overload , which is associated with its inhi-bition of TLR4, NF-κB and TNF-αin myocardium ex-pression .
ABSTRACT
Objective To study the effects of CUEDC2 on renal interstitial fibrosis and inflammation response in rats with unilateral ureteral obstruction (UUO). Methods 30 Balb/c rats were randomly distributed into sham operation group(sham-vector),uuo operation group(uuo-vector) and CUEDC2 treatment group after uuo (uuo-cuedc2). Hematoxylin-eosin and Masson staining were used to measure renal pathology; Inflammation factors were quantified by ELISA; Immunohistochemistry was performed to measure the expression of CUEDC2;Protein expression of CUEDC2, Fibronectin, E-cadherin, Collagen I were detected by Western Blot. Results At 7 and 14d after operation, the area of interstitial fibrosis and expression of ICAM1,MCP1,IL1,IL8, Fibronectin and Collagen I in uuo-cuedc2 showed a marked decrease when compared to uuo-vector (p?0.05),the level of E-cadherin was significantly increased (P < 0.05). Conclusion CUEDC2 can inhibit renal interstitial fibrosis and decrease the expression of inflammation factors and Collagen deposition.
ABSTRACT
Objective To analyze the number of spleen B-cells and the expression of inflammatory factors and sirtuin 1 (SIRT1) in spleen B-cells of CD38-/- mice, so as to explore the effects of CD38 gene knockout on inflammatory factors in B-cells and its potential mechanism. Methods The DNA levels of CD38 and Neo gene in mouse tail tissues were detected by polymerase chain reaction (PCR). Spleen B-cells from wide-type (WT) C57BL/6 and CD38-/-mice were sorted by magnetic activated cell sorting (MACS), and the purity of sorting B-cells were identified by flow cytometry. The mRNA levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and CD38 gene were detected by real-time PCR, the protein expressions of CD38 and SIRT1 were detected by Western blotting analysis. Results We confirmed the successful establishment of CD38-/-mice and sorted spleen B-cells from WT and CD38-/-mice (purity>95%). Compared with WT mice, the development of spleen was hampered in the CD38-/-mice, the number of spleen cells and spleen B-cells were significantly reduced (P<0. 01), the mRNA levels of TNF-α and IL-1β were significantly decreased (P<0. 01), and the expression level of SIRT1 was significantly increased in CD38-/-mice (P<0. 05). Conclusion CD38 gene knockout can reduce the number of B-cells in the spleen; and it can inhibit the expression of inflammatory factors (TNF-α and IL-1β) in spleen B-cells by activating SIRT1 pathway.
ABSTRACT
Objective To explore the correlation between insulin resistance,inflammation factors and cognitive dysfunction in patients with metabolic syndrome (MS).Methods 150 cases of patients with MS were chosen,and they were divided into group A1 (n=68) and group A2 (n=82) according to whether they were with mild cognitive dysfunction (MCI).50 healthy cases chosen from physical examination were set as group B.The general data,laboratory examination results and inflammatory factor levels were compared between each group.MCI was set as dependent variable,insulin sensitivity index(ISI),high sensitive C-reactive protein(hs-CRP),and tumor necrosis factor-α(TNF-α) were set as independent variables.The relationship between inflammatory factor,insulin resistance and MCI was analyzed with multiple factors logistic regression analysis.Results The difference of body mass index(BMI),total cholesterol (TC),triglyceride (TG),low density lipoprotein (LDL),highdensity lipoprotein (HDL),fasting blood glucose(FBG),hemoglobin A1c (HbA1c),fasting insulin (FINS),and ISI between the three groups had statistical significance (P＜0.05).The levels of HbAlc in group A1 and group A2 were significantly higher than those in group B (P＜0.05).FBG and FINS showed a trend of reduce among the three groups,while ISI showed a trend of increase.The difference had statistical significance between each group (P＜0.05).The difference of hs-CRP,TNF-α between the three groups had statistical significance (P＜0.05).Levels of hs-CRP and TNF-α in group A1 were significantly higher than those in group A2 and group B,while the levels of hs-CRP and TNF-α in A2 group was significantly higher than those in group B (P＜0.05).Point biserial correlation analysis showed that the risk of MCI for MS patients had positive correlation with FBG,FINS,hs-CRP and TNF-α(r=0.219,0.231,0.309 and 0.287,P＜0.05),while it had negative correlation(r=-0.294,P＜0.05) with ISI.Multiariable logistic regression analysis showed that the more severe of insulin resistance (the lower ISI),the higher of inflammatory factor hs-CRP and TN-o,the greater the risk of MCI in patients with MS.Conclusion Insulin resistance and inflammation degree are more obvious in patients with MS combined with MCI,which can predict risk of cognitive dysfunction in patients with MS.
ABSTRACT
Objective: To study the protective effect of Zhenlong Xingnao Capsule (ZXC) on the cerebral ischemia-reperfusion injury of rats. Methods: Total 120 Sprague-Dawley (SD) rats were randomly divided into Sham operation group, the middle cerebral artery occlusion (MCAO) model group, Nimodipine (37 mg/kg BW) group, low-dose ZXC (125 mg/kg) group, and high-dose ZXC (250 mg/kg). A focal cerebral ischemia-reperfusion model was made by using the occlusion of MCAO and occlusion of the right middle cerebral artery in rats. The infarct volume and the neurological deficit were determined by TTC staining and Longa's score; And to determine the contents of GSH, T-AOC, T-SOD, and MDA in left and right half brain tissues. To detect the protein expression level changes of P38, NF-κB, Bcl-2, Bax, and Caspase-3 by Western blotting method. Results: Compared with the Sham operation group, the neurologic symptom scores and infarct volume of MCAO of rats in model group were significantly increased, and the vigor of T-AOC and T-SOD was decreased. At the same time the protein expression of P38 and Caspase-3 was promoted. Compared with the model group, the neurologic symptom scores, injury degree of brain tissues, and brain infarct volume of rats with cerebral ischemia-reperfusion injury were significantly decreased in the Nimodipine, low-dose ZXC, and high-dose ZXC groups, the vigor of T-SOD and T-AOC was increased. The high-dose ZXC can inhibit P38 and Caspase-3 expressions. Conclusion: ZXC has the obvious protective effect on the brain ischemia-reperfusion injury in rats. Its mechanism may be related to the improvement of neurological function, the reduction of free radical injury, the inhibition of inflammation and the cell apoptosis.
ABSTRACT
Objective To study effect of triple viable bacterium of bacillus (small peifeikang) on the intestinal microflora and inflammation factors in patients with infectious diarrhea.Methods From October 2010 to October 2014, 120 patients with infectious diarrhea were chosen as study objects, 30 cases of health examination were selected as healthy control group, All patients were divided into 2 groups:60 patients in small peifeikang group and 60 patients in control group.The therapeutic effect and the change of intestinal microflora and inflammation factors were compared between two groups.Results The effective rate of small peifeikang groupwas 100.00%, while that of control group was 96.67%,the difference had statistical significance (χ2 =5.52,P<0.05).The symptom disappearance time of diarrhea, vomiting and fever had statistical difference between two groups (P<0.05), that of dehydration had no statistical significance.After treatment, in small peifeikang group and control group, the number of bifidobacterium, lactobacillus, and B/E value had decreased significantly (P<0.05), and the number of intestinal bacteriahad increased significantly(P<0.05).The distribution of intestinal flora in small peifeikang group was better than that of control group, and the difference had statistical significance(P<0.05).After treatment, in small peifeikang group and control group, the level of endotoxin, IL -6, TNF-αand CRP had decreased significantly( P <0.05).The extent of decreasing in small peifeikang group was more significant than that of control group, and the difference had statistical significance ( P <0.05 ) . Conclusion The triple viable bacterium of bacillus can promote the recurrence of patients with infectious diarrhea by adjusting the distribution of intestinal microflora, and by its immune regulation function.
ABSTRACT
Objective To explore the effect of intensive statin on platelet activity and inflammation factors 24 h after rat myocar‐dial infarction .Methods Seventy Sprague‐Dawley rats were randomly divided into five groups (n= 14):Sham‐operated group (SHAM group);AMI group(control group) ,general group;intensive statin therapy group ;general and intensive statin therapy group;established AMI rat model by ligation of left anterior descending branch of coronary artery .The general group ,general and intensive statin therapy group was fed atorvastatin by 10 mg · kg -1 · d-1 with distilled water 2 mL by intragastric gavage daily for two weeks .The intensive statin therapy group ,general and intensive statin therapy group was fed atorvastatin by 50 mg/kg with distilled water 2 mL by intragastric gavage 12 h before surgery .Serum PAC‐1 ,CD62p ,TNF‐α,hs‐CRP was measured at the time of 24 h of postoperation .Results TNF‐α,hs‐CRP ,PAC‐1 and CD62p levels in control group were significantly higher than the SHAM group and intensive statin group 24 h after the LADS were ligated(P 0 .05);and there was no significant difference between normal group and control group in all the four factors (P>0 .05) .Conclusion Intensive statin therapy before acute myocardial infarction could decrease the level of inflammation factors and inhibit platelet activity postop‐eration .
ABSTRACT
Objective To investigate the influence of Xiyanping injections therapy on inflammation factors and lung function for old patients with acute exacerbation chronic obstructive pulmonary disease (AECOPD).Methods Ninety-two patients with AECOPD were randomly divided into the control group (n =46) and observation group(n =46).Patients in control group were given conventional treatment,in observation group were given XiYanPing injections for 2 weeks treatment on the basis of treatment of control group.The C-reactive protein(CRP),blood sedimentation rate (ESR),interleukin 10 (IL-10),1 s forced expiratory volume (FEV1),forced vital capacity (FVC),FEV1/FVC change and clinical effect of the two groups were recorded.Results There was no statistically significant difference in terms of CRP,ESR and IL-10 between the two groups before treatment (P ＞ 0.05).After treatment,CRP and ESR in control group were reduced from (35.6 ±9.8) mg/L and (56.2 ± 16.4) mm/h to (14.2 ± 8.1) mg/L and (22.4 ± 11.6) mm/1 h and the difference was significant (t =-14.515,-12.008;P ＜ 0.05),while in observation group decreased from (36.4 ± 8.2) mg/L,(54.6 ± 15.4) to (8.2 ± 6.2) mg/L,(16.8 ± 9.6) mm/h and the difference was significant (t =-18.468,-15.249;P ＜ 0.05).Meanwhile,the level of IL-10 in the two groups significantly increased and the observation group showed more.IL-10 in control group increased from (168.8 ± 62.4) ng/L to (212.4 ± 85.6) ng/L and the difference was significant(t =5.468,P ＜ 0.05),and in observation group increased from (172.8 ± 65.1) ng/L to (266.4 ± 92.6) ng/L and the difference was significant (t =6.368,P ＜ 0.05).The FEV1 and FVC were markedly increased after treatment and the observation group showed increase more.FEV1and FVC in control group were increased from (1.41 ±0.15) L and (2.62 ±0.88) L to (1.62 ± 0.18) L and (2.89 ± 0.92) L,and the differences were significant (t =2.186,2.415 ; P ＜ 0.05).While in observation group,FEV1 and FVC were increased from (1.42 ± 0.16) L and (2.59 ± 0.84) L to (1.84 ±0.21) L and (3.12 ± 0.94) L,and the differences were significant (t =4.366,4.585 ; P ＜ 0.05).Total efficacy of observation group was 91.3％ (42/46),obviously higher than that of control group(78.3％ (36/46) ; x2 =4.658 ;P ＜ 0.05).Conclusion Xiyanping injections can reduce the inflammatory response in patients with AECOPD,improve lung function and the effect of treatment.
ABSTRACT
Objective To study the influence of microinflammation factors on the pregnancy outcome in gestational diabetes mellitus (GDM) patients.Methods Forty-eight GDM women with abnormal hsCRP and IL-6 concentration were selected as study group another 36 GDM women with normal concentration as control group.The difference of the two groups in common data and outcomes of pregnancy were retrospectively analyzed.Results There were significant difference in hypertensive disorders,prom,premature delivery,intra-uterine asphyxia,mega fetus,uterine-incision delivery and fetus hypoglycemia hypocalcemia between two groups (P ＜0.05).Conclusions Microinflammation factors have promote influence on the development and outcomes of GDM.Early diagnosis and impose intervention may have positive effect.